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Image Search Results
Journal: Oncoimmunology
Article Title: Severe, but not mild heat-shock treatment induces immunogenic cell death in cancer cells
doi: 10.1080/2162402X.2017.1311433
Figure Lengend Snippet: DCs pulsed with sHS-treated tumor cells stimulated antigen-specific CD4+ T cells and induced higher CD8+ T cell activation and proliferation. DCs incubated with sHS or mHS-treated cells for 24 h were added to autologous T lymphocytes at ratio 1:10. The number of IFNγ producing (A) CD8+ and (D) CD4+ T cells, proliferation (Ki67) and activation (CD137) of (C) CD8+ and (D) CD4+ T cells was determined after 7 d after one round of restimulation. (B) The number of MP158–66-specific CD8+ T cells was determined after 9 d. (E) Percentage of Foxp3+CD127low T regulatory cells from CD4+CD25+ T cells. Dotplots are representative of 4–8 donors. Graph shows percentage of IFNγ+CD8+ T cells or IFNγ+CD4+ T cells from CD8+ or CD4+ T cells, respectively and represents means ± SEM of 4–8 donors (*p < 0.05). (F) Immunoblot of tumor antigens after HS treatment. Immunoblots are representative of n = 3.
Article Snippet: Antibodies The following antibodies were used for human studies: anti-human Calreticulin, HSP90 (both from Enzo Life Sciences), HSP70 (R&D Systems), CD47 (B6H12; Santa Cruz Biotechnology), anti-CD80-FITC, CD86-PE, CD83-PerCP-Cy5.5 (Beckman Coulter, Inc.), HLA-DR-PE-Cy7, IFNγ-FITC (BD Biosciences), CD11c-APC, CD8-PE-Dylight594, CD3-Alexa 700, CD8-Alexa Fluor 700, CD127-Alexa647, CD45RO-APC (ExBio),
Techniques: Activation Assay, Incubation, Western Blot