anticd4 pe cy7 antibody Search Results


90
Becton Dickinson apc-cy7–conjugated anti-cd4
Apc Cy7–Conjugated Anti Cd4, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Thermo Fisher mouse fitc-anti-pd1
Mouse Fitc Anti Pd1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Thermo Fisher anti-cd4-pe-cy7
Anti Cd4 Pe Cy7, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Thermo Fisher anti-cd4 pe-cy7
Anti Cd4 Pe Cy7, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Quantum Dot Inc monoclonal antibodies to mouse cd4 qdot-605
Monoclonal Antibodies To Mouse Cd4 Qdot 605, supplied by Quantum Dot Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Thermo Fisher foxp3-alexa488 antibody
DCs pulsed with sHS-treated tumor cells stimulated antigen-specific <t>CD4+</t> T cells and induced higher CD8+ T cell activation and proliferation. DCs incubated with sHS or mHS-treated cells for 24 h were added to autologous T lymphocytes at ratio 1:10. The number of IFNγ producing (A) CD8+ and (D) CD4+ T cells, proliferation (Ki67) and activation (CD137) of (C) CD8+ and (D) CD4+ T cells was determined after 7 d after one round of restimulation. (B) The number of MP158–66-specific CD8+ T cells was determined after 9 d. (E) Percentage of Foxp3+CD127low T regulatory cells from CD4+CD25+ T cells. Dotplots are representative of 4–8 donors. Graph shows percentage of IFNγ+CD8+ T cells or IFNγ+CD4+ T cells from CD8+ or CD4+ T cells, respectively and represents means ± SEM of 4–8 donors (*p < 0.05). (F) Immunoblot of tumor antigens after HS treatment. Immunoblots are representative of n = 3.
Foxp3 Alexa488 Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Becton Dickinson anti-cd4-apc/cy7
DCs pulsed with sHS-treated tumor cells stimulated antigen-specific <t>CD4+</t> T cells and induced higher CD8+ T cell activation and proliferation. DCs incubated with sHS or mHS-treated cells for 24 h were added to autologous T lymphocytes at ratio 1:10. The number of IFNγ producing (A) CD8+ and (D) CD4+ T cells, proliferation (Ki67) and activation (CD137) of (C) CD8+ and (D) CD4+ T cells was determined after 7 d after one round of restimulation. (B) The number of MP158–66-specific CD8+ T cells was determined after 9 d. (E) Percentage of Foxp3+CD127low T regulatory cells from CD4+CD25+ T cells. Dotplots are representative of 4–8 donors. Graph shows percentage of IFNγ+CD8+ T cells or IFNγ+CD4+ T cells from CD8+ or CD4+ T cells, respectively and represents means ± SEM of 4–8 donors (*p < 0.05). (F) Immunoblot of tumor antigens after HS treatment. Immunoblots are representative of n = 3.
Anti Cd4 Apc/Cy7, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Thermo Fisher anticd4 pe cy7
DCs pulsed with sHS-treated tumor cells stimulated antigen-specific <t>CD4+</t> T cells and induced higher CD8+ T cell activation and proliferation. DCs incubated with sHS or mHS-treated cells for 24 h were added to autologous T lymphocytes at ratio 1:10. The number of IFNγ producing (A) CD8+ and (D) CD4+ T cells, proliferation (Ki67) and activation (CD137) of (C) CD8+ and (D) CD4+ T cells was determined after 7 d after one round of restimulation. (B) The number of MP158–66-specific CD8+ T cells was determined after 9 d. (E) Percentage of Foxp3+CD127low T regulatory cells from CD4+CD25+ T cells. Dotplots are representative of 4–8 donors. Graph shows percentage of IFNγ+CD8+ T cells or IFNγ+CD4+ T cells from CD8+ or CD4+ T cells, respectively and represents means ± SEM of 4–8 donors (*p < 0.05). (F) Immunoblot of tumor antigens after HS treatment. Immunoblots are representative of n = 3.
Anticd4 Pe Cy7, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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86
Danaher Inc rabbit anti cd4 cy7
DCs pulsed with sHS-treated tumor cells stimulated antigen-specific <t>CD4+</t> T cells and induced higher CD8+ T cell activation and proliferation. DCs incubated with sHS or mHS-treated cells for 24 h were added to autologous T lymphocytes at ratio 1:10. The number of IFNγ producing (A) CD8+ and (D) CD4+ T cells, proliferation (Ki67) and activation (CD137) of (C) CD8+ and (D) CD4+ T cells was determined after 7 d after one round of restimulation. (B) The number of MP158–66-specific CD8+ T cells was determined after 9 d. (E) Percentage of Foxp3+CD127low T regulatory cells from CD4+CD25+ T cells. Dotplots are representative of 4–8 donors. Graph shows percentage of IFNγ+CD8+ T cells or IFNγ+CD4+ T cells from CD8+ or CD4+ T cells, respectively and represents means ± SEM of 4–8 donors (*p < 0.05). (F) Immunoblot of tumor antigens after HS treatment. Immunoblots are representative of n = 3.
Rabbit Anti Cd4 Cy7, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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SouthernBiotech cd4 pe-cy7 ct-4; mouse igg1k
DCs pulsed with sHS-treated tumor cells stimulated antigen-specific <t>CD4+</t> T cells and induced higher CD8+ T cell activation and proliferation. DCs incubated with sHS or mHS-treated cells for 24 h were added to autologous T lymphocytes at ratio 1:10. The number of IFNγ producing (A) CD8+ and (D) CD4+ T cells, proliferation (Ki67) and activation (CD137) of (C) CD8+ and (D) CD4+ T cells was determined after 7 d after one round of restimulation. (B) The number of MP158–66-specific CD8+ T cells was determined after 9 d. (E) Percentage of Foxp3+CD127low T regulatory cells from CD4+CD25+ T cells. Dotplots are representative of 4–8 donors. Graph shows percentage of IFNγ+CD8+ T cells or IFNγ+CD4+ T cells from CD8+ or CD4+ T cells, respectively and represents means ± SEM of 4–8 donors (*p < 0.05). (F) Immunoblot of tumor antigens after HS treatment. Immunoblots are representative of n = 3.
Cd4 Pe Cy7 Ct 4; Mouse Igg1k, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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93
Cytek Biosciences pe cy7 anti cd4
DCs pulsed with sHS-treated tumor cells stimulated antigen-specific <t>CD4+</t> T cells and induced higher CD8+ T cell activation and proliferation. DCs incubated with sHS or mHS-treated cells for 24 h were added to autologous T lymphocytes at ratio 1:10. The number of IFNγ producing (A) CD8+ and (D) CD4+ T cells, proliferation (Ki67) and activation (CD137) of (C) CD8+ and (D) CD4+ T cells was determined after 7 d after one round of restimulation. (B) The number of MP158–66-specific CD8+ T cells was determined after 9 d. (E) Percentage of Foxp3+CD127low T regulatory cells from CD4+CD25+ T cells. Dotplots are representative of 4–8 donors. Graph shows percentage of IFNγ+CD8+ T cells or IFNγ+CD4+ T cells from CD8+ or CD4+ T cells, respectively and represents means ± SEM of 4–8 donors (*p < 0.05). (F) Immunoblot of tumor antigens after HS treatment. Immunoblots are representative of n = 3.
Pe Cy7 Anti Cd4, supplied by Cytek Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


DCs pulsed with sHS-treated tumor cells stimulated antigen-specific CD4+ T cells and induced higher CD8+ T cell activation and proliferation. DCs incubated with sHS or mHS-treated cells for 24 h were added to autologous T lymphocytes at ratio 1:10. The number of IFNγ producing (A) CD8+ and (D) CD4+ T cells, proliferation (Ki67) and activation (CD137) of (C) CD8+ and (D) CD4+ T cells was determined after 7 d after one round of restimulation. (B) The number of MP158–66-specific CD8+ T cells was determined after 9 d. (E) Percentage of Foxp3+CD127low T regulatory cells from CD4+CD25+ T cells. Dotplots are representative of 4–8 donors. Graph shows percentage of IFNγ+CD8+ T cells or IFNγ+CD4+ T cells from CD8+ or CD4+ T cells, respectively and represents means ± SEM of 4–8 donors (*p < 0.05). (F) Immunoblot of tumor antigens after HS treatment. Immunoblots are representative of n = 3.

Journal: Oncoimmunology

Article Title: Severe, but not mild heat-shock treatment induces immunogenic cell death in cancer cells

doi: 10.1080/2162402X.2017.1311433

Figure Lengend Snippet: DCs pulsed with sHS-treated tumor cells stimulated antigen-specific CD4+ T cells and induced higher CD8+ T cell activation and proliferation. DCs incubated with sHS or mHS-treated cells for 24 h were added to autologous T lymphocytes at ratio 1:10. The number of IFNγ producing (A) CD8+ and (D) CD4+ T cells, proliferation (Ki67) and activation (CD137) of (C) CD8+ and (D) CD4+ T cells was determined after 7 d after one round of restimulation. (B) The number of MP158–66-specific CD8+ T cells was determined after 9 d. (E) Percentage of Foxp3+CD127low T regulatory cells from CD4+CD25+ T cells. Dotplots are representative of 4–8 donors. Graph shows percentage of IFNγ+CD8+ T cells or IFNγ+CD4+ T cells from CD8+ or CD4+ T cells, respectively and represents means ± SEM of 4–8 donors (*p < 0.05). (F) Immunoblot of tumor antigens after HS treatment. Immunoblots are representative of n = 3.

Article Snippet: Antibodies The following antibodies were used for human studies: anti-human Calreticulin, HSP90 (both from Enzo Life Sciences), HSP70 (R&D Systems), CD47 (B6H12; Santa Cruz Biotechnology), anti-CD80-FITC, CD86-PE, CD83-PerCP-Cy5.5 (Beckman Coulter, Inc.), HLA-DR-PE-Cy7, IFNγ-FITC (BD Biosciences), CD11c-APC, CD8-PE-Dylight594, CD3-Alexa 700, CD8-Alexa Fluor 700, CD127-Alexa647, CD45RO-APC (ExBio), CD4-PE-Cy7, Foxp3-Alexa488, CD3-PerCP-Cy5.5, (eBioscience), CD137-PE (4–1BB), CD25-PerCP-Cy5.5, Ki-67-PE (BioLegend).

Techniques: Activation Assay, Incubation, Western Blot